(downloadable reprints available in PDF format)


The biophysical basis for phosphorylation-enhanced DNA-binding autoinhibition of the ETS1 transcription factor.
Perez-Borrajero, C., Okon, M., Sheng-Huei, C., Scheu, K., Graves, B.J., Murphy, M.E.P., and McIntosh, L.P.

J. Mol. Biol. 431: 595-614 (2019)   

The pKa values of the catalytic residues in the retaining glycoside hydrolase T26H mutant of T4 lysozyme.
Brockerman, J.A., Okon, M., Withers, S.G., and McIntosh, L.P.

Protein Science 28: 620-632 (2019)   


Modulating the nucleophile of a glycoside hydrolase through site-specific incorporation of fluoroglutamic acids.
Kötzler, M.P., Robinson, K., Chen, H.-M., Okon, M., McIntosh, L.P., and Withers, S.G.

J. Am. Chem. Soc. 140: 8268-8276 (2018)   

Characterization of the two conformations adopted by the T3SS inner-membrane protein PrgK.
Bergeron, J.R.C., Brockerman, J.A., Vuckovic, M., Deng, W., Okon, M., Finlay, B.B., McIntosh, L.P., and Strynadka, N.C.J.

Protein Science 27: 1680-1691 (2018)   

Biophysical characterization of the tandem FHA domain regulatory module from the Mycobacterium tuberculosis ABC transporter Rv1747.
Heinkel, F., Shen, L., Richard-Greenblatt, M., Okon, M., Bui, J.M., Gee, C.L., Gay, L.M., Alber, T., Av-Gay, Y., Gsponer, J., and McIntosh, L.P.

Structure 26: 972-986 (2018)   

The voltage-gated sodium channel EF-hands forms an interaction with the III-IV linker that is disturbed by disease-causing mutations.
Gardill, B.R., Rivera-Acevedo, R.E., Tung, C.-C., Okon, M., McIntosh, L.P., and Van Petegem, F.

Scientific Reports 8: 4483 (2018)   


ETV4 and AP1 transcription factors form multivalent interactions with three sites on the MED25 activator-interacting domain.
Currie, S.L., Doane, J.J., Evans, K.S., Bhachech, N., Madison, B.J., Lau, D.K.W., McIntosh, L.P., Skalicky, J.J., Clark, K.A., and Graves, B.J.

J. Mol. Biol. 429: 2975-2995 (2017)   

Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase.
Kötzler, M.P., McIntosh, L.P., and Withers, S.G.

Protein Science 26: 1555-1563 (2017)   

Structured and disordered regions cooperatively autoinhibit the DNA binding of ETV1, ETV4, and ETV5.
Currie, S.L., Lau, D.K.W., Doane, J.J., Whitby, F.G., Okon, M., McIntosh, L.P., and Graves, B.J.

Nucleic Acids Res. 45: 2223-2241 (2017)   

Discovery and characterization of small molecules targeting the ETS factor ERG in prostate cancer.
Butler, M.S., Roshan-Moniri1, M., Hsing, M., Lau, D., Kim, A., Yen, P., Mroczek, M., Nouri, M., Lien, S., Axerio-Cilies, P., Dalal, K., Yau, C., Ghaidi, F., Guo, Y., Yamazaki, T., Lawn, S., Gleave, M., Gregory-Evans, C.Y., McIntosh, L.P., Cox, M.E., Rennie, P.S., and Cherkasov, A.

Oncotarget 8: 42438-42454 (2017)   


Conformational dynamics and the binding of specific and non-specific DNA by the autoinhibited transcription factor Ets-1.
Desjardins, G., Okon, M., Graves, B.J., and McIntosh, L.P.

Biochemistry 55: 4105-4118 (2016)   

Structural and dynamics studies of Pax5 reveal asymmetry in domain stability and DNA binding by the Paired domain.
Perez-Borrajero, C., Okon, M., and McIntosh, L.P.

J. Mol. Biol. 428: 2372-2391 (2016)   

Autoinhibition of DNA binding by ETV6 is established by the stability of its inhibitory helix.
De, S., Okon, M., Graves, B.J., and McIntosh, L.P.

J. Mol. Biol. 424: 1515-1539 (2016)   


Arginine: Its pKa value revisited.
Fitch, C., Platzer, G., Okon, M., Garcia-Moreno, B., and McIntosh, L.P.

Protein Science 24: 7525-761 (2015)   

The modular structure of the inner membrane ring component PrgK facilitates assembly of the type III secretion system basal body.
Bergeron, J.R.C., Worrall, L.J., De, S., Sgourakis, N.G., Cheung, A.H., Lameignere, E., Okon, M., Wasney, G., Baker, D., McIntosh, L.P., and Strynadka, N.C.J.

Structure 23: 161-172 (2015)   


pH dependent 1H, 13C, and 15N chemical shifts of the ionizable amino acids: a guide for protein pKa measurements.
Platzer, G., Okon, M., and McIntosh, L.P.

J. Biomol. NMR 60: 109-129 (2014) (suppl. material)  

Structural characterization of the C3 domain of cardiac myosin binding protein-C and its hypertrophic cardiomyopathy-related R502W mutant.
Zhang, L., De, S., McIntosh, L.P., and Paetzel, M.

Biochemistry 53: 5332-5342 (2014) (suppl. material)  

Synergy of aromatic residues and phosphoserines within the intrinsically disordered DNA-binding inhibitory elements of Ets-1.
Desjardins, G., Meeker, C.A., Bhachech, N., Currie, S.L., Okon, M., Graves, B.J., and McIntosh, L.P.

Proc. Natl. Acad. Sci. USA 111: 11019-11024 (2014)   

O-GlcNAc modification of tau directly inhibits its aggregation without perturbing the conformational properties of tau monomers.
Yuzwa, S.A., Cheung, A.H., Okon, M., McIntosh, L.P., and Vocadlo, D.J.

J. Mol. Biol. 426: 1736-1752 (2014) (suppl. material)  

Detection and characterization of serine and threonine hydroxyl protons in Bacillus circulans xylanase by NMR spectroscopy.
Brockerman, J.A., Okon, M., and McIntosh, L.P.

J. Biomol. NMR 58: 17-25 (2014) (suppl. material)   


Steric mechanism of auto-inhibitory regulation of specific and non-specific DNA binding by the ETS transcriptional repressor ETV6.
De S., Chan A.C.K., Coyne H.J. III, Bhachech, N., Helmsdorf, U., Okon, M., Murphy M.E., Graves, B.J., and McIntosh, L.P.

J. Mol. Biol. 426: 1390-1406 (2013) (suppl. material)  

Protein dielectrc constants determined from NMR chemical shift perturbations.
Kukic P., Farrell D., McIntosh L.P., Garcia-Moreno B., Jensen K., Toleikia Z., Teilum K., and Nielsen J.E.

J. Am. Chem. Soc. (2013) (suppl. material)  

Mutation of salt bridge-forming residues in the ETV6 SAM domain polymer interface blocks ETV6-NTRK3 induced cellular transformation.
Catinbas, N., Huang-Hobbs, H., Tongon, C., Leprivier, G., An, J., Bowdon, M., Chow, C., Gleave, M., McIntosh, L.P., and Sorensen, P.H.B.

J. Biol. Chem. 288: 27940-17950 (2013)  

Strategies for modulating the pH-dependent activity of a family 11 glycoside hydrolase.
Ludwiczek, M.L., D'Angelo, I., Yalloway, G.N., Brockerman, J.A., Okon, M., Nielsen, J.E., Strynadka, N.C., Withers, S.G., and McIntosh, L.P.

Biochemistry 52: 3138-3156 (2013) (suppl. material)  

Structure-function analysis of a broad specificity Populus trichocarpa endo-β-glucanase reveals an evolutionary link between bacterial licheninases and plant XTH gene products.
Eklöf J.M., Shojani, S., Okon, M., McIntosh, L.P., and Brummer, H.

J. Biol. Chem. 288: 15786-15799 (suppl. material)  

Investigating the structural dynamics of α-1,4-galatosyltransferase LgtC from Neisseria meningitidis by nuclear magnetic resonance spectroscopy.
Chan, P.H.W., Cheung, A.H., Okon, M., Chen, H.-M., Withers, S.G., and McIntosh, L.P.

Biochemistry 51: 8278-8292 (2012) (suppl. material)  

1H, 13C, and 15N resonance assignments and peptide binding site chemical shift perturbation mapping for the Escherichia coli redox enzyme chaperone DmsD.
Stevens, C.M., Okon, M., McIntosh, L.P., and Paetzel, M.

Biomol. NMR Assign. 7: 193-197 (2013)   


Nuclear magnetic resonance spectral assignments of α-1,4-galactosyltransferase LgtC from Neisseria meningitidis: substrate binding and multiple conformational states.
Chan, P.H.W., Weissbach, S., Okon, M., Withers, S.G., and McIntosh, L.P.

Biochemistry 51: 8278-8292 (2012) (suppl. material)  

The PNT domain from Drosophila Pointed-P2 contains a dynamic helix preceded by a disordered phosphoacceptor sequence.
Lau, D.K.W., Okon, M., and McIntosh, L.P.

Protein Sci. 21: 1716-1725 (2012) (suppl. material)  

The zinc regulated antivirulence pathway of Salmonella is a multiprotein immunoglobin adhesion system.
Prehna, G., Li, Y., Stoynov, N., Okon, M., Vuckovic, M., McIntosh, L.P., Foster, L.J., Findlay, B.B., and Strynadka, N.C.

J. Biol. Chem. 287: 32324-32337 (2012)

Auto-inhibition of ETV6 (TEL) DNA-binding: Appended helices sterically block the ETS domain.
Coyne, H.J. III, De, S., Okon, M., Green, S., Bhachech, N. Graves, B.J., and McIntosh, L.P.

J. Mol. Biol. 421: 67-84 (2012) (suppl. material)  

A plecksterin homology-related domain in SHIP1 mediates membrane localization during Fcγ receptor induced phagocytosis.
Ming-Lum, A., Shojani, S., So, E., McCarrell, E., Shaw, E., Vu, D., Wang, I., McIntosh, L.P., and Mui, A. L.

FASEB J. 26: 3163-3177 (2012)

A protein export pathway involving Escherichia coli porins.
Prehna, G., Zhang, G., Gong, X., Dusyk, M., Okon, M., McIntosh, L.P., Weiner, J.H., and Strynadka, N.C.J.

Structure 20: 1154-1155 (2012) (suppl. material)  

Putting a Stop to D,L-transpeptidases.
De, S. and McIntosh, L.P.

Preview for Structure 20:753-754 (2012)

Regulation of mitosis and taxane response by Daxx and Rassf1C.
Giovinazzi, S., Lindsay, C.R., Morozov, V.M., Escobar-Cabrera, E., Summers, M.K., Han, H.S., McIntosh, L.P., and Ishov, A.

Oncogene 31: 13-26 (suppl. material)   


Structure, dynamics, and ionization equilibria of the tyrosine residues in Bacillus circulans xylanase.
Baturin, S.J., Okon, M. and McIntosh, L.P.

NMR 51: 379-394 (2011) (suppl. material)  

Dissecting electrostatic interactions in Bacillus circulans xylanase through NMR-monitored pH titrations.
McIntosh, L.P., Natio, D., Baturin, S.J., Okon, M., Joshi, M.D., and Nielsen, J.E.

J. Biomol. NMR 51: 5-19 (2011) (suppl. material)  

Genomic and biochemical insights into the specificity of ETS transcription factors.
Hollenhorst, P., McIntosh, L.P. and Graves, B.J.

Ann. Rev. Biochem. 80: 437-471 (2011) (suppl. material)

Characterizing the N- and C-terminal SUMO interacting motifs of the scaffold protein DAXX.
Escobar-Cabrera, E., Okon, M., Lau, D.K., Dart, C.F., Bonvin, A.M., and McIntosh, L.P.

J. Biol. Chem. Mar 7 286: 19816-19829 (2011) (suppl. material)  

Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Webb, H., Tynan-Connolly, B.M., Lee, G.M., Farrell, D., O'Meara, F., Søndergaard, C.R., Teilum, K., Hewage, C., McIntosh, L.P., Nielsen, J.E.

Proteins 79: 685-702 (2011)

Structural characterization of Escherichia coli BamE, a lipoprotein component of the β-barrel assembly machinery complex.
Kim, K.H., Kang, H.-S., Okon, M, Escobar-Cabrera, E., McIntosh, L.P., Paetzel, M.

Biochemistry 50: 1081-1090 (2011)   


Structural characterization of the DAXX N-terminal helical bundle domain and its complex with Rassf1C.
Escobar-Cabrera, E., Lau, D.K.W., Giovinazzi, S., Ishov, A.M., McIntosh, L.P.

Structure 18: 1642-1653 (2010) (suppl. material)

Ras signal requires dynamic properties of Ets1 for phosphorylation-enhanced binding to co-activator CBP.
Nelson, M.L., Kang, H.-S., Lee, G.M., Blaszczak, A.G., Lau, D.K.W., McIntosh, L.P., and Graves, B.J.

Proc. Natl. Acad. Sci. 107: 10026-10031 (2010) (suppl. material)

DNA binding by the ETS protein TEL (ETV6) is regulated by autoinhibition and self-association.
Green, S.M., Coyne III, H.J., McIntosh, L.P., and Graves, B.J.

J. Biol. Chem. 285: 18496-18504 (2010) (suppl. material)

The anti-invasive peptide Neopetrosiamide A induces the release of membrane-bound vesicles and inhibits tumor cell adhesion.
Austin, P., Heller, M., Williams, D.E., McIntosh, L.P., Vogl, W., Foster, L.J., Andersen, R.J., Roberge, M., and Roskelly, C.D.

PLoS One 5: e10836 (2010)

Circular permutation of Bacillus circulans xylanase: a kinetic and structural study.
Reitinger, S., Yu, Y., Wicki, J., Ludwiczek, M., D'Angelo, I., Baturin, S.J., Okon, M., Strynadka, N.C.J., Lutz, S., Withers, S.G., and McIntosh, L.P.

Biochemistry 49: 2464-2474 (2010)

Titration_DB: Storage and analysis of NMR-monitored protein pH titration curves.
Farrell, D., Saja-Miranda, E., Webb, H., Georgi, N., Crowley, P., McIntosh, L.P. and Nielsen, J.E.

Proteins 78: 843-857 (2010) (suppl. material)   


NMR spectroscopic characterization of the Sialyltransferase CstII from Camplyobacter jejuni: Histidine 188 is the general base.
Chan, P.H.W., Lairson, L.L., Lee, H.J., Wakarchuk, W.W., Strynadka, N.C.J., Withers, S.G., and McIntosh, L.P.

Biochemistry 48: 11220-11230 (2009)

Detection and assignment of phosphoserine and phosphothreonine residues by 13C-31P spin-echo difference NMR spectroscopy.
McIntosh, L.P., Kang, H.-S., Okon, M., Nelson, M.L., Graves, B.J., and Brutscher, B.

J. Biomol. NMR 43: 31-37 (2009)   


Structural characterization of the type III pilotin-secretin complex from Shigella flexneri.
Okon, M., Moraes, T., Lario, P.I., Creagh, A. L., Haynes, C. A., Strynadka, N.C.J., and McIntosh, L.P.

Structure 16: 1544-1554 (2008) (suppl. material)

The crystal structure of MexR from Pseudomonas aeruginosa in complex with its anti-repressor ArmR.
Wilke, M., Heller, M., Creagh, A.L., Haynes, C.A., McIntosh, L.P., Poole, K., and Strynadka, N.C.J.

Proc. Natl. Acad. Sci. 105: 14831-14837 (2008). (suppl. material)

The affinity of Ets-1 for DNA is modulated by phosphorylation through transient interactions of an unstructured region.
Lee, G.M., Pufall, M.A., Meeker, C.A,, Kang, H.S., Graves, B.J., and McIntosh, L.P.

J. Mol. Biol. 382: 1014-1030 (2008) (suppl. material)

Phage display and crystallographic analysis reveals potential substrate / binding site interactions in the protein secretion chaperone CsaA from Agrobacterium tumefaciens.
Feldman, A.R., Shapova, Y.A., Wu, S.S.T., Oliver, D.C., Heller, M., McIntosh, L.P., Scott, J.K., and Paetzel, M.

J. Mol. Biol. 379: 457-470 (2008) (suppl. material)

Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABPα.
Kang, H.-S., Nelson, M.L. Mackereth, C.D., Schärpf, M., Graves B.J., and McIntosh, L.P.

J. Mol. Biol. 377: 636-646 (2008) (suppl. material)

Determination of electrostatic interaction energies and protonation state populations in enzyme active sites.
Søndergaard, C. R., McIntosh, L.P., Pollastri, G., and Nielsen, J.E.

J. Mol. Biol. 376: 269-287 (2008) (suppl. material)   


A secondary xylan binding site enhances the catalytic activity of a single domain family 11 glycoside hydrolase.
Ludwiczek, M.L., Heller, M., Kantner, T., and McIntosh, L.P.

J. Mol. Biol. 373: 337-354 (2007) (suppl. material)

Peptide binding by a fragment of calmodulin composed of EF-hands 2 and 3.
Lakowski, T.M., Lee, G.M., Lelj-Garolla, B., Okon, M., Reid, R.E., and McIntosh, L.P.

Biochemistry 46: 8525-8536 (2007)

Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: Histidine characterization by NMR spectroscopy.
Schubert, M., Poon, D.K.Y., Wicki, J., Tarling, C.A., Kwan, E.M., Nielsen, J.E., Withers, S.G., and McIntosh, L.P.

Biochemistry 46: 7383-7395 (2007) (suppl. material)

Calcium-induced folding of a fragment of calmodulin composed of EF-hands 2 and 3.
Lakowski, T.M., Lee, G.M., Okon, M., Reid, R.E., and McIntosh, L.P.

Prot. Sci. 16: 1119-1132 (2007)

Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi xylanase Cex through NMR spectroscopic analysis.
Poon, D.K.Y., Withers, S.G. and McIntosh, L.P.

J. Biol. Chem. 282: 2091-2100 (2007) (suppl. material)

NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate.
Poon, D.K.Y., Ludwiczek, M.L., Schubert, M., Kwan, E.M., Withers, S.G. and McIntosh, L.P.

Biochemistry 46: 1759-1770 (2006) (suppl. material)   


Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy.
Poon, D.K.Y., Schubert, M., Au, J., Okon, M., Withers, S.G. and McIntosh, L.P.

J.A.C.S. 128: 15388-15389 (2006) (suppl. material)  

Beads-on-a-String, characterization of Ets-1 sumoylated within its flexible N-terminal sequence.
Macauley, M.S., Errington, W.J., Scharpf, M., Mackereth, C.D., Blaszczak, A.G., Graves, B.J., and McIntosh, L.P.

J. Biol. Chem. 281: 4164-4172 (2006) (suppl. material)  

Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosidase allows the structural investigation of the parent enzyme/substrate complex.
Kim, Y.-W., Lovering, A.L., Chen, H., Kantner, T., McIntosh, L.P., Strynadka, N.C.J., and Withers, S.G.

J. Am. Chem. Soc. 128: 2202-2203 (2006) (suppl. material) 

Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi.
Kim, Y.-W., Fox, D.T., Hekmat, O., Kantner, T., McIntosh, L.P., Warren, R.A.J., and Withers, S.G.

Org. Biomol. Chem. 4: 2025-2032 (2006)   


Dissecting the domain structure of Cdc4p, a mysoin essential light chain involved in Schizosaccharomyces pombe cytokinesis.
Escobar-Cabera E., Venkatesan, M., Desaytels, M., Hemmingsen, S.M., McIntosh, L.P.

Biochemistry 44: 12136-12148 (2005) (suppl. material)  

Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region.
Pufall, M.A., Lee, G.M., Nelson, M.L., Kang, H.-S., Velyvis, A., Kay, L.E., McIntosh, L.P., and Graves, B.J.

Science 309: 142-145 (2005) (suppl. material)  

The structural and dynamic basis of Ets-1 DNA binding autoinhibition.
Lee, G.M., Donaldson, L.W., Pufall, M.A., Kang, H.-S., Pot, I., Graves, B.J., and McIntosh, L.P.

J. Biol. Chem. 280: 7088-7099 (2005) (suppl. material)  

Backbone chemical shift assignments of the LexA catalytic domain in its active conformation.
Okon, M., Pfuetzner, R.A., Vuckovic, M., Little, J.W., Strynadka, N.C.J., and McIntosh, L.P.

J. Biomol. NMR, 31: 371-372 (2005)   


Cyclic enterobacterial common antigen: Potential contaminant of bacterially expresed protein preparations.
Erbel, P.J.A., Seidel, R., MacIntosh, S.E., Gentile, L.N., Amor, J.C., Kahn, R.A., Prestegard, J.H., McIntosh, L.P., and Gardner, K.H.

J. Biomol. NMR 29: 199-204 (2004)

Mutations in the SAM domain of the ETV6-NTRK3 chimeric tyrosine kinase block polymerization and transformation activity.
Tognon, C.E., Mackereth, C.D., Somasiri, A.M., McIntosh, L.P., and Sorensen, P.H.B.

Mol. Cell. Biol. 24: 4636-4650 (2004) 

Structural characterization of the RNase E S1 domain and identification of its oligonucleotide-binding and dimerization interfaces.
Schubert, M., Edge, R.E., Lario, P., Cook, M.A., Strynadka, N.C.J., Mackie, G.A., and McIntosh, L.P.

J. Mol. Biol. 341: 37-54 (2004) (suppl. material) 

Diversity in structure and function of Ets family PNT domains.
Mackereth, C.D., Scharpf, M., Gentile, L.N., MacIntosh, S.E., Slupsky, C.M., and McIntosh, L.P.

J. Mol. Biol. 342: 1249-1264 (2004) (suppl. material) 

Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1.
Macauley, M.S., Errington, W.J., Okon, M., Scharpf, M., Mackereth, C.D., Schulman, B.A., and McIntosh, L.P.

J. Biol. Chem. 279: 49131-49137 (2004) (suppl. material)   


Characterizing the pH-dependent stability and catalytic mechanism of the family 11 xylanase from the alkalophilic Bacillus agaradhaerens.
D.K.Y. Poon, P. Webster, S.G. Withers and L.P. McIntosh.

Carb. Res. 338: 415-421 (2003)   


Mechanism of E47-Pip Interaction on DNA Resulting in Transcriptional Synergy and Activation of  Immunoglobulin Germline Sterile Transcripts. 
S. Nagulapalli, A. Goheer, L. Pitt, L.P. McIntosh and M.L. Atchison.

Mol. Cell. Biol. 22: 7337-7350 (2002)

Chemical shift and secondary structure conservation of the PNT/SAM domains from the Ets family of transcription factors.
C.D. Mackereth, M. Schärpf, L.N. Gentile and L. P. McIntosh

J. Biomol. NMR 24: 71-72 (2002)

Oligomerization-dependent association of the SAM domains from Schizosaccharomyces pombe Byr2 and Ste4.
R. Ramachander, C.A. Kim, M.L. Phillips, C.D. Mackereth, C.D. Thanos, L.P. McIntosh and J.U. Bowie.

J. Biol. Chem. 277: 39585-39593 (2002)

Site-specific characterization of the association of Xylooligosaccharides with the CMB13 Lectin-Like Xylan-binding domain from Streptomyces lividans Xylanase 10A by NMR Spectroscopy.
M. Schärpf, G. P. Connelly, G. M. Lee, A. B. Boraston,  R. A. Warren and L. P. McIntosh

Biochemistry 41: 4255-4263 (2002)

An NMR approach to structural proteomics.
A. Yee, X. Chang, A. Pineda-Lucenda, B. Wu, A. Semesi, B. Le, T. Ramelot, G. M. Lee, S. Bhattacharyya, P. Guttierez, A. Denisov, C.-H. Lee, J. Cort, G. Koslov, J. Liao, L. Chen, D. Wishart, W. Lee, L. P. McIntosh, K. Gehring, M.A. Kennedy, A. M. Edwards, and C. H. Arrowsmith

Proc. Natl. Acad. Sci. 99: 1825-1830 (2002)

The solution structure and interactions of CheW form Thermotoga Maritima.
I. J. Griswold, H. Zhou, R. V. Swanson, L. P. McIntosh, M. I. Simon, and F.W. Dahlquist

Nature Struct. Biol. 9:121-125 (2002)

Autoinhibition allosterically regulates Ets-1 DNA binding through a dipole driven phosphate contact.
H. Wang, L. P. McIntosh, and B. J. Graves

J. Biol.Chem. 277: 2225-2233 (2002)   


NMR-based structure of the conserved protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum. G.M. Lee, A.M. Edwards, C.H. Arrowsmith, and L.P. McIntosh

J. Biomol. NMR 21: 63-66 (2001) (suppl. material)

Assignment of selectively 13C-labeled cellopentaose synthesized using an enginered glycosynthase.
E. Brun, H. Brumer III, L.F. MacKenzie, S. G. Withers, and L.P. McIntosh

J. Biomol. NMR 21: 67-68 (2001)

Dissecting the electrostatic interactions and pH-dependent activity of a Family 11 Glycosidase.
M.D. Joshi, G. Sidhu, J.E. Nielsen, G.D. Brayer, S.G. Withers, and L.P. McIntosh

Biochemistry 40, 10115-10139 (2001) (suppl. material)

Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe.
C.M. Slupsky, M. Desautels, T. Huebert, R. Zhao, S.M. Hemmingsen and L.P. McIntosh

J. Biol. Chem. 276, 5943-5951 (2001)

Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe, interacts with a phosphatylinositol 4-kinase.
M. Desautels, J.P. Den Haese, C.M. Slupsky, L.P. McIntosh and S.M. Hemmingsen

J. Biol. Chem. 276, 5932-5942 (2001)   


Structural proteomics of an archeon.
D. Christendat, A. Yee, A. Dharamsi, Y. Kluger, A. Savchenko, J.R. Cort, V. Booth, C.D. Mackereth, V. Saridakis, I. Ekiel, G. Kozlov, K.L. Maxwell, N. Wu, L.P. McIntosh, K. Gehring, M.A. Kennedy, A.R. Davidson, E.F. Pai, M. Gerstein, A.M. Edwards and C.H. Arrowsmith

Nature Struct. Biol. 7, 903-909 (2000)

Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
C.D. Mackereth, C.H. Arrowsmith, A.M. Edwards and L.P. McIntosh

Proc. Natl. Acad. Sci. USA 97(12) 6316-6321 (2000)

Hydrogen bonding and catalysis: A novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.
M.D. oshi, G. Sidhu, I. Pot, G.D. Brayer, S.G. Withers and L.P. McIntosh

J. Mol. Biol. 299, 255-279 (2000)

Structure and binding specificity of the second N-terminal cellulose-binding domain from Cellulomonas fimi endoglucanaseC.
E. Brun, P.E. Johnson, A.L. Creagh, P. Tomme, P. Webster, C.A. Haynes and L.P. McIntosh

Biochemistry 39, 2445-2458 (2000) (suppl. material)

Binding site analysis of cellulose-binding domain CBD_N1 from endoglucanase C of Cellulomonas fimi by site-directed mutagenesis.
J. Kormos, P.E. Johnson, E. Brun, P. Tomme, L.P. McIntosh C.A. Haynes and D.G. Kilburn

Biochemistry 39, 8844-8852 (2000)

Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate.
G.P. Connelly, S.G. Withers and L.P. McIntosh

Protein Science 9, 512-524 (2000)   


The highly conserved beta-haripin of the paired DNA-binding domains is required for the assembly of Pax:Ets ternary complexes.
W. Wheat, H. Lennox, D. Fitzsimmons, S.J. Krautkramer, L.N. Gentile, L.P. McIntosh and J. Hagman

Mol. Cell. Biol. 19, 2231-2241 (1999)

The cellulose-binding domains from Cellulomonas fimi CenC beta-1,4-glucanase bind nitroxide spin-labeled cellooligosaccharides in multiple orientations.
P.E. Johnson, E. Brun, L. Mackenzie, S.G. Withers and L.P. McIntosh

J. Mol. Biol. 287, 609-625 (1999) (suppl. material)

Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
G. Sidhu, S.G. Withers, N.T. Nguyen, L.P. McIntosh, L. Ziser and G.D. Brayer

Biochemistry 38, 5346-5354 (1999)   


Characterization of a buried neutral histidine in Bacillus circulans xylanase: internal dynamics and interaction with a bound water molecule.
G.P. Connelly and L.P. McIntosh

Biochemistry 37, 1810-1818 (1998)

Binding and stablility of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC.
A.L. Creagh, J. Koska, P.E. Johnson, P. Tomme, M.D. Joshi, L.P. McIntosh, D.G. Kilburn and C.A. Haynes
Biochemistry 37, 3529-3535 (1998)

Assigning the NMR spectra of aromatic amino acids in proteins: analysis of two Ets pointed domains.
C.M.Slupsky, L.N. Gentile and L.P. McIntosh

Biochemistry and Cell Biology (Thematic Issue on Biomedical NMR in Canada) 76, 379-390 (1998)

Calcium binding by The N-terminal cellulose-binding domain from Cellulomonas fimi beta-1,4-glucanase CenC.
P.E. Johnson, A.L. Creagh, E. Brun, K. Joe, P. Tomme, C.A. Haynes and L.P. McIntosh

Biochemistry 37, 12772-12781 (1998)

Structure of the Ets-1 Pointed domain and MAP kinase phosphorylation site.
C.M. Slupsky, L.N. Gentile, L.W. Donaldson, C.D. Mackereth, J.J. Seidel, B.J. Graves, and L.P. McIntosh

Proc. Natl. Acad. Sci. USA 95, 12129-12134 (1998)   


Stereospecific assignments of the NH2 resonances from the primary amides of asparagine and glutamine side-chains in isotopically labeled proteins, L.P. McIntosh, E. Brun and L.E.J. Kay

J. Biomol. NMR 9, 306-312 (1997)

High Resolution NMR structure and backbone dynamics of the Bacillus subtilius response regulator, Spo0F.
V.A. Feher, J.W. Zapf, J.A. Hoch, J.M. Whiteley, L.P. McIntosh, M. Rance, N.J. Skelton, F.W. Dahlquist, J. Cavanagh

Biochemistry, 36, 10015-10025 (1997)

Specific 15N, NH correlations for residues in 15N, 13C fractionally deuterated proteins that immediately follow methyl-containing amino acids.
D.R. Muhandiram, P.E. Johnson, D. Yang, O. Zhang, L.P. McIntosh and L.E. Kay

J. Biomol. NM. 10, 283-288 (1997)

Complete measurement of the pKa values of the carboxyl and imidazole groups in Bacillus circulans xylanase.
M.D. Joshi, A. Hedberg and L.P. McIntosh

Protein Science 6, 2667-2670 (1997)   


Solution structure of the murine Ets-1 ETS domain: a winged helix-turn-helix DNA binding protein.
L.W. Donaldson, J.P. Petersen, B.J. Graves and L.P. McIntosh

EMBO J. 15, 125-134 (1996)

Structural coupling of the inhibitory regions flanking the ETS domain of murine Ets-1.
J.J. Skalicky, L.W. Donaldson, J.M. Petersen, B.J. Graves and L.P. McIntosh

Protein Science 5, 296-309 (1996)

The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of Bacillus circulans xylanase.
L.P. McIntosh, G. Hand, P.E. Johnson, M.D. Joshi, M. Körner, L.A. Plesniak, L. Ziser, W.W. Wakarchuk and S.G. Withers

Biochemistry 35, 9958-9966 (1996)

Interaction of soluble celloliogosaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 2. NMR and ultraviolet absorbance spectroscopy.
P.E. Johnson, P. Tomme, M.D. Joshi and L.P. McIntosh

Biochemistry 35, 13895-13906 (1996)

Secondary structure and NMR assignments of Bacillus circulans xylanase. L.A. Plesniak, W.W. Wakarchuk and L.P. McIntosh

Protein Science 5, 1118-1135 (1996)

Characterization of a buried neutral histidine in Bacillus circulans xylanase: NMR assignment, pH titration, and hydrogen exchange.
L.A. Plesniak, G.P. Connelly, W.W. Wakarchuk and L.P. McIntosh

Protein Science 5, 2319-2328 (1996)

Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by NMR spectroscopy.
P.E. Johnson, M.D. Joshi, P. Tomme, D.G. Kilburn and L.P. McIntosh

Biochemistry 35, 14381-14394 (1996) (suppl. material)

ETS domain DNA binding.
B.J. Graves, M.E. Gillespie and L.P. McIntosh

Nature (Scientific Correspondence) 384, 322 (1996)

Probing the role of tryptophan residues in a cellulose-binding protein by chemical modifications.
M.R. Bray, P.E. Johnson, N.R. Gilkes, L.P. McIntosh, D.G. Kilburn, and R.A. Warren

Protein Science 5, 2311-2318 (1996)   


Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an alpha-helix.
J.P. Petersen, J.J. Skalicky, L.W. Donaldson, L.P. McIntosh, T. Alber and B.J. Graves

Science 269, 1866-1869 (1995)

Structural features of the Escherichia coli ATP synthase (ECF1Fo) from NMR spectroscopy.
Wilkens, S., Dahlquist, F.W., McIntosh, L.P., Donaldson, L.W. and Capaldi, R.A.

Nature Structure Biology 2: 961-967 (1995)   


The secondary structure of the ETS domain places murine Ets-1 in the superfamily of winged helix-turn-helix DNA-binding proteins.
Donaldson, L.W., Petersen, J.M., Graves, B.J. and McIntosh, L.P.

Biochemistry 33: 13510-13516 (1994)